Coding

Part:BBa_K587001:Design

Designed by: David Ignacio Maycotte Cervantes   Group: iGEM11_ITESM__Queretaro   (2011-09-28)


RecA protein coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of RecA was changed from its original source, because there was a EcoRI site in the middle of the DNA sequence so the sequence chaged in one base to modify that restriction site.


Source

The information of this sequence comes from the article: Cleavage of the X and P22 Repressors by recA Protein by Robert T. Sauer, Michael J. Ross, and Mark Ptashnel from the Journal of Biological Chemistry. Obtained from the Escherichia coli CFT073 chromosome, complete genome from NCBI

References